Turn pump to ‘On’ 7. Vacuum degassing: the solvent is exposed to a vacuum and the reduced pressure removes more than 60% of the dissolved air. 0 • August 2022 *4820. Change the pump program back to 50/50 water/ACN to re-equilibrate the system e. If a purge valve is not functioning correctly, it can lead to the vehicle losing power or stalling unexpectedly. Turn off the pump by pressing "Pump" on the LC-20AD 4. 8 Click pump on the flow diagram and change the flow path into A2 and B2. The 1260 Infinity II Flexible Pump combines high performance and simplified operation with highest flexibility in automated gradient formation and solvent blending. Preparing the Binary Pump 51 Algae Growth in HPLC Systems 52 Setting up the Pump with the Instrument Control Interface 54 Priming the Pump 64 Solvent Information 66. For long-term storage, it is best to follow manufacturer’s recommendations. Connect its other end to a waste container. Use only HPLC-grade solvents and water filtered through 0. Figure 6: Pump head with purge valve and outlet to autosampler. We routinely see HPLC systems with white fluffy crystals built up around the pump heads, pistons and various fittings from lack of maintenance on a daily basis. Overall Routine Pump Maintenance 1. With Arc HPLC System, you can easily replicate and improve the performance of your existing LC methods without compromising data quality eliminating the burdens of older, less efficient LC systems. 4411-EN Revision 2. Move to step 2. Air or particulates in head of pump or valves of the pump Leakage in transmission lines Solution • Avoid formation of negative pressure, utilisation of equalising valves in the caps of the eluent flask, prepare fresh buffer • Check tightness, if necessary, replace seals or valves • Purge the pumps with water or IPA, flush waterWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. a Continue to purge for a short while. 5 mL/min c. The system must be primed to remove air bubbles and for the pump to function properly. Make sure that you know which solvent is linked to which pump. To purge the 2414 via Empower, use the Purge. ), prime system if necessary. Replacing the Plunger Seal and Plunger. Maintaining Your Agilent 1100 Series HPLC System Optimize performance and maximize efficiency in your lab. Click Okay c. , 5-10 mL/min. • Flush the entire system flow path with water for at least 10 min at 2 mL/min. If the preceding step does not solve the issue, remove the purge valve and start the pump. . Priming and Purging the 1525 Binary HPLC Pump. RCM (right click mouse) in the Quat Pump module i. the solvent flow of the HPLC If equipped with a ballast valve check that the ballast valve on the rough pump is . See your pump modules user manual for detailed instructions on re-assembly. ResearchGate | Find and share researchpump for extended seal lifetime † Setting upper and lower pressure limits will turn off the pump, e. Make sure that you know which solvent is linked to which pump. Otherwise, locate where the increased pressure is coming from. 998. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. 5:26. Pump 15 minutes at this pressure to wear in the seals. keep the system at this flow rate for about 10 minutes to equilibrate the system without the Column. They are available in various configurations (→ Supported Configurations, page 5) and can thus be used in numerous laboratory environments. 15 mL/min. So, let's get started and purge away any confusion! Purging, in the context of lab equipment, is the process of flushing a lot of. categories. If your problem is. 7. • Press “Menu” till purge appears on screen. Priming and Purging the 1525 Binary HPLC Pump. The name of the game in HPLC is repeatability. Purge Line Figure 1 322 HPLC Pump. - filter non-HPLC grade solvents - filter buffer solutions - Install an in-line filter between auto-sampler and column (removes pump seal debris, ALS rotor debris, and sample particulates). the solvent flow of the HPLC If equipped with a ballast valve check that the ballast valve on the rough pump is . Blocked channels can often be released by purging with a high flow with warm water (60 to 70 °C, HPLC grade). HPLC system. Excellent gradient performance resulting from the highly accurate solvent delivery by the 5110/5160 pump with Hitachi’s unique high-speed. Pump & Maintenance Kits for HPLC Part Number: 01018-22707. ) If system has check valve, loosen valve to allow air to escape. To manually purge the reference side of the 2414 flow cell, press the Shift + 1 button (Purge) on the screen while the pump is flowing mobile phase. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is down to zero; Analysis. This video shows the proper procedure to safely remove and replace the plunger seal on the Shimadzu LC-40 Series Nexera pump. A pressure sensor monitors the pump pressure. 8. Waters recommends storing used columns in 100% acetonitrile and avoiding long-term storage in buffered eluents. 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. Label bottles correctly with bottle content,. For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide. Secondary Pump Head Parts 216 Purge Valve 220 Cover Parts 222 Leak Parts 224 Fuses 224 Accessory Kit 225 Tools 226 HPLC System Tool Kit 228. Attach syringe to prime/purge valve. Use only HPLC-grade solvents and water filtered through 0. 06% or 0. 45 µm inert hydrophilic filter (avoid cellulose) and degas all eluents thoroughly, using sonication under vacuum. Your purge valve is a great tool for flushing and priming your HPLC pump system. Discover the efficient LC-40D pump for routine HPLC applications up to 44 MPa (440 bar. closed. The descriptions in this manual apply to the LPG-3400XRS pump of the UltiMate™ 3000 series. Seal could have fluoropolymer O-ring energizer instead (black O-ring). Initially, the pressure was unstable. 11 Click everything on. Press the purge buttons again to. 5 um frit for 1. HPLC pumps are designed to pump liquid, not – Improve Peak Shape for Ionizable Compounds air. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. Wash the system with isopropanol (excluding the column). This can be caused by reduced fuel and airflow in the system, resulting in misfires and power loss. A flow rate of 5mL/min is commonly used for standard HPLC systems. After purging the pump heads, pu rge the ProFlow flow meter for about 10 minutes. 0:44. Priming and Purging the 1525 Binary HPLC Pump. Solving Problems That Matter - Bioprocessing. Therefore, to estimate how much volume is required for a purge, check the volume specification of the inlet tubing, the degasser and the pump that you are using, then double it to give an approximate purge volume. Fig 2. At this point, the mobile phase is ready for analysis. Consumables likely need to be replaced on aqueous pump. In such case clogging of the column is most likely happening due to: additive (i. Turn off the pump flow and wait until the system pressure is down to zero. Open the manual purge valve or use the purge command,. Before using an HPLC system, you should prime all of the lines in your HPLC pump. Kata kunci / keywords: HPLC/HPLC instrument, metode analisis/analysis method, metode isolasi/ isolation method, bahan kimia alam/ natural chemical product. If you've ever wondered how to purge this piece of equipment, you're in the right place. The Logi-CHROM pump is compact yet powerful featuring a high quality HPLC pumping system. The system must be primed to remove air bubbles and for the pump to function properly. Between samples when your compound is very sticky (apolar) to help clean the column. 5 01. The 1260 Infinity II Binary Pump is ideally suited for a broad range of UHPLC applications using columns with conventional, superficially porous or sub-2-micron particles. HPLC Solvent Management Waters HPLC pumps enable you to customize HPLC Systems to accommodate your solvent delivery needs, whether for isocratic or gradient, analytical or preparative. First statement: Don’t let it run dry. Figure 6: Pump head with purge valve and outlet to autosampler. The historical evolution of HPLC pumps and their characteristics has been reviewed in many textbooks (1–3), as well as journal articles (4–6). If pressure fluctuations are still. the purge valve, and the actual pump mechanism. The name of the game in HPLC is repeatability. In general, the degasser should be left on, and all solvent lines including the unused ones should be filled with solvents. This will redirect the solvent flow to the waste exit of the valve instead of passing through the whole system. You may need to remove and sonicate it. Replace the O-Ring on the Purge Valve 150 Remove the Pump Head Assembly 153 Maintenance of a Pump Head Without Seal Wash Option 155HPLC troubleshooting guide Introduction During the development of a new HPLC method, there were unexpected results—all peaks, except for the. 02 to 0. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. Is the pump fuse in working order?Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. Performing the Ramp and Decay Test After a PM. 9. Disconnect your column first and fill your mobile phase bottle with IPA (isopropyl alcohol) start your pump. 1ml/min2 g. ID stainless steel tubing kit • Galaxie Chromatography Software-0. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. Search by lot number or partial lot number. From my little experience i learnt that it may be due to air trapped in. In a low-pressure gradient (LPG) pump type, a proportioning valve is used to aspirate the desired solvent mix. Perform search. Using a solvent filtration kit or an online degasser will remove any dissolved. The source of column clogging comes from particulates in a number of places:We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Turn . BEFORE WORKING WITH THIS ANALYTICAL HPLC 1. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. PROCEDURE: Flush the system using one of the cleaning mixtures as described in the cleaning. An optional seal wash function is available for applications using Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact Solving Problems That Matter - Batteries. Prime the pump with degassed solvent. 2. Prepare the Pump • Use fresh or different mobile phase (as required). The display will show, 515 Pump, 100 µl, Ver 1. ) If system has check valve, loosen valve to allow air to escape. 3:36. Solvent mixing: The proportionating valve (lower-front of image) allows the desired volume ratio of the different solvents to be drawn from the reservoirs. . Purge valve Purge function Remove the purge valve waste line and the capillary from the purge valve outlet. A damping unit is connected between the two piston chambers. Thermo Scientific Dionex UltiMate 3000 Series Nano/Cap System NCS-3500RS Nano/Cap Pump NCP-3200RS Operating Instructions (Original Operating Instructions)This instalment is the first of a series of four white papers on high performance liquid chromatography (HPLC) modules (pump, autosampler, UV detector, and chromatography data system) to be published in 2019. In fire and explosion protection engineering, an inert (ie, non-flammable) purge gas (like nitrogen, helium, argon, etc) is introduced into an enclosed system (eg, a container or process vessel) to prevent the formation of a fuming. Their pulse-free solvent flow, from analytical to semi-preparative flow rates, makes them ideal for use with highly sensitive detectors. It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound. Make sure to confirm that the purge valve isHow to prevent your HPLC from running dry. Check for leaks and perform PM. Leak at pump 18 Selectivity Differences in selectivity 19 Baseline Disturbance at void time 20. For more information, visit tutorial demonstrates how to prime the pumps in Bio-Rad's new NGC liquid chromatography sy. Solving Problems That Matter - Bioprocessing. Purging pump and autosampler for i-Series Step 4. Figure 7: Pump tab. GasesUltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB DGP-3600A, DGP-3600AB,. The 1290 Infinity II High Speed Pump has an exceptionally low delay volume, which allows you to run ultrafast. If seal wash option is installed, replace wash seals and gaskets. New InletWeaver using proven Jet Weaver technology The 1290 Infinity Quaternary Pump offers the highest accuracy andAir or particulates in head of pump or valves of the pump Leakage in transmission lines Solution • Avoid formation of negative pressure, utilisation of equalising valves in the caps of the eluent flask, prepare fresh buffer • Check tightness, if necessary, replace seals or valves • Purge the pumps with water or IPA, flush waterFirmenich Dubai. Catalog number: 5040. The user manual for the Agilent G1311A Quaternary Pump, a high-performance liquid chromatography (HPLC) module that provides reliable gradient formation and precise flow rates. Purge pump and attempt to clean check valves. From my little experience i learnt that it may be due to air trapped in. Popular answers (1) There can be several reasons for the pressure drops you observe: air could still be present in the system after your recent repair (i. Before using an HPLC system, you should prime all of the lines in your HPLC pump. 7 Repeat steps 4-6 with the next 3 valves. Vanquish Binary Pump N: Up to 1500 bar (22,000 psi) and 100 µL/min with active flow control. degasser ports and/or pump head multichannel valve or pump head inlet, as needed, to draw liquid through until all lines are filled. Purge the Pump (whenever changing buffers) a. Algae Growth in HPLC Systems 81 Prevent Blocking of Solvent Filters 82 Normal Phase Applications 83 4 Optimizing Performance 85 Using the Degasser 86. The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then to VERIFY that each channel delivers the reported amount of fluid to the column (measure it). Check for leaks and perform PM. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. If the VP button does not show mobile phase, press “CE” followed by “Function” until “System” is shown on the screen. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Mobile phase is passed through porous polymer tubing placed in a vacuum chamber. In other words is the system configuration comprising of the pump, injector/autosampler, column, and detector is appropriate for carrying out the analysis in. 5 01. Prepare the Pump • Use fresh or different mobile phase (as required). ID stainless steel tubing kit • Galaxie Chromatography Software-0. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. g. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting solvent of your application at a reasonable flow rate to rinse out any other solvent; Install the appropriate HPLC column for your application; Close all doors of the. In the right part of the image, the glass piston is. Remove and clean pistons. Use the keypad to type in the solvent composition for pumps A, B, C, and D. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. PROCEDURE: Flush the system using one of the cleaning mixtures as described in the cleaning. Search. If problem persists, flush system with 100% methanol or isopropanol. L/mni for 5mni . 18: Ready Check Results window appears after the flow meter purge function is If the conclusions from the article How to Troubleshoot no flow or no pressure issues: Binary/Quaternary/Isocratic Pumps - G131XX, and G711XX, and performing the Pump Leak Rate Test, points to a defective or clogged AIV, you may try the following procedure: How to Sonicate the Inlet and Outlet Valves of Agilent 1100, 1200 and 1260 Series Pumps. This will prevent any flushed contaminants from getting lodged into the latter components of the system. It provides HPLC systems with an automatic, continuous method of removing dissolved gases from mobile phases. Purging air from pump system: Change solvent line to a bottle containing 100% isopropanol. A key element of its user-friendly functionality is the accessibility of the system for. Stop the pump and shut down software “HPL1(online)” f. This pump enables quaternary solvent delivery at pressures up to 800. If using MassLynx or Empower. Close the cover. Email Priming and Purging a 1525 Binary Series HPLC Pump to a friend ; Read More. g. When the purge valve is open, solvent from the quaternary pump will go straight to waste rather than proceeding through the column. 1. Draw syringe back to prime. Performing the Ramp and Decay Test After a PM. 2:19. off. Dear Huq, check out the whole tubing starting from Pump to the Column inlet for any air trapped, purge the pump for few minutes. HPLC maintenance tutorial on how to change the High Pressure Filter on an Agilent. Residues or contaminations may block filters or capillaries. 1 A) Calibration of Pump:-. Label bottles correctly with bottle content, and filling date / expiry date. See Reference 1 for interactive video. Removing dissolved gases from the mobile phase improves the performance andSo that is how you fix an air bubble in the pump. The 1525 and 1525 with EF heads are integrated, high-pressure, binary HPLC pumps that feature on-board pulse dampening and efficient mixing. The manual. (1ml/min) to 4 or 5 hour. Therefore, the term "the Purge pump and attempt to clean check valves. some time this may be due to filters in the channels; please remove your filters (a inlet filter from your solvent reservoir bottle to the HPLC pump ) and sonicate in the Methanol. Fig 2. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. The technical specifications of all pumps meet the highest. Contacting Us Vanquish Pumps (VH-P10) Operating Manual Page 3 Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales. Purge valve open. Overlay of 6 pump proportioning chromatograms in 10% gradient. • Vacuum chambers – Contain the gas-permeable eluent channel. 1-15 of 17. 0. Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. HPLC is an abbreviation for High Performance Liquid Chromatography. For modular autosamplers purge from front panel. Leaking pump heads Check the seals and replace, if necessary. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. The EVAP system is designed to prevent fuel vapors from escaping into the atmosphere by capturing and temporarily storing them in a. 400 bar max. g. |The 1290 Infinity II Bio Flexible Pump. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. - Troubleshooting the degasser always needs to be linked with troubleshooting the mobile phase. The system will start the countdown from 5 minutes to zero. ISET enables. Close the purge valve. • Switch “ON” the HPLC Pump. 3601-EN Revision 5. The PTFE frit is very accessible and easy to switch out, too. If problem still persists, contact system manufacturer. Pump 100% isopropanol through the pump for 3 minutes. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Changing from one operating pump head to the other you simply move the yellow connection tube to the other pump head outlet. Problem 3:Inconsistent or too slow pump flow rate. It is column chromatography. purge is complete, enter a flow of 1 mL/min and 100% B. For reverse-phase columns, wet the column using a mobile phase like methanol and bring it to normal flow rate. A = 100%). 9-0. During step 1, check that the solvent is freely moving through the solvent lines. c. Peak shape C. However, in order to obtain a full understanding of the pump, Thermo Fisher Scientific recommends that you review the manual thoroughly before beginning operation. If I run the system at 0. Yes it looks like this! Now the valve is ok and there’s no more leak, maybe now the frit is soaked by the mobile phase and fits better?! Thanks!! reply from dmarchini< dmarchini >…. Eluent may then be allowed to progress through the IC system, and the pressure monitored. 2. That could help to reduce air bubble generation. b. Liquid Chromatography (LC) Troubleshooting Resources. . . Use the keypad to type in the solvent composition for pumps A, B, C, and D. Typically a 0. 3. Flush the column with 10/90 water/ACN for 5 min at 1. Request Information. A peristaltic pump (piezo pump) delivers fresh wash solvents to the fl ush port. I use a reverse phase Waters HPLC. , 5-10 mL/min. (You can press the same bottoms again to stop purging. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting. 2 Push the “conc” button on LC20AD. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline. Rebuilding the Reference Valve. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is zero; Analysis. HPLC Principle. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies courses. 13), a precisely defined quantity of air (“the gas ballast”) is admitted into the pumping chamber of the pump. Make sure that the AVR7-3 inject valve is in the Purge position. then you have a little bit of work to do to really prime the line as that pump is not really powerful enough to draw in liquid if it is super dry. Manually prime using a priming syringe: Insert the priming syringe into the Luer fitting at the center of the draw-off valve handle. Wash out the blockage. ), prime system if necessary. Press the EDIT/ ENTER key to start purging. 0*Today, we're diving into the exciting world of lab consumables and equipment, specifically focusing on the Shimadzu HPLC pump. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. 2. Set maximum flow gradient to 0. 8 3. Perform sample clean-up (i. The Vanquish HPLC and UHPLC systems offer uncompromised performance, robustness, and ease of use. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. Achieve binary-like performance in your HPLC instrument. thermoscientific Vanquish UHPLC and HPLC Systems Operating Manual 4820. If problem persists, flush system with 100% methanol or isopropanol. Moreover, if the faulty purge valve completely blocks the engine, it cannot start or run. 5 um columns, use 0. Evaluate the results; Stop the batch; Apply a corrective pump purge; Re-equilibrate the system; Restart the batch Nexera looks for evidence of flow. Run each pump for 2 minutes. HPLC pumps can be single, binary, or ternary, meaning that the can make use of one, two, or more different solvent reservoirs. See "Pressure pulsation or inconstant pressure" in Pressure on page 120. Equal functions for Right Pump found just below. Replace the part that causes the obstruction. Pump Valves for HPLC. Open the purge valve and set the flow rate of 5 mL/min for each channel with HPLC grade water. Rebuilding the Reference Valve. Detector – responds to the separated analytes emerging from the HPLC. Always do this one channel at a time (e. 8. Close the purge valve, set the flow to a value that gives a pressure of 350 bar. HPLC pumps incorporate single or dual piston, syringe, or diaphragm pump designs. For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ Dionex™ Ultimate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. 2. Air bubbles can create such flow anomalies. The sensor signals the control board when the vacuum is below a preset level. degasser ports and/or pump head multichannel valve or pump head inlet, as needed, to draw liquid through until all lines are filled. Flow rate range of 0. 5 Close the purge valve, set the flow to a rate adequate to achieve a pressure of 350 bar. Pump 15 min at this pressure to wear the seals in. [][]Clean the solvent diffuser/filter once in a week. 1:55. Note: Follow the instructions in the Ready Check Results window and close both purge screws. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. 2. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. Flushing and purging all solvent lines and fluidics is the first step that should be done if unstable system pressure. Operating principle. 결과 및 정리. Purging the solvent pumps 1. 1-15 of 17. g. Purification Solvent Management. Then press “Enter”, “1”, “Enter”, “CE”, “CE”. , 10 mL/min. The binary Semipreparative High-Pressure Gradient P680 Pump is ideal for small-scale purification applications. ANSWER: The purge solvent is the solvent being pushed from the pump. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. Open the black purge valve and press the “Purge” button. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. c Enter the new parameters in step 8, and click OK. Preparation steps: Flush the pump head with an appropriate solvent using the purge function. Greetings. Select this procedure when you want to prime and purge any 1525 Binary Series. It features an integrated pressure sensor, which ensures pressure-independent flow rate accuracy. Leak at fittings, purge valve, active inlet If there are still signs of a. The Agilent 1260 Infinity II Flexible Pump is a quaternary UHPLC system pump that provides gradient formation and solvent blending from up to four solvents. Choose between 3 configurations: A 2 × 2 binary high pressure gradient (HPG) for high accuracy blending of up to two eluents from two selectable solvents each. Maintaining your LC columns. Repeat twice after the cycle has finished after approximately 4 min. Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head UltiMate 3000 Series: Pump Series Operating Instructions Page I Declaration of Conformity (Original Declaration) Product: UltiMate 3000 Series Pump Types: LPG-3400AB, LPG-3400M and LPG-3400MB For stainless steel flow paths, proceed to Step 6; For PEEK flow paths, the cleaning procedure is completed. This is needed to purge any air from the tubing, introduce fresh mobile phase to. You may. 2 to 0. Remove and disassemble the pump head. SPE, LLE) on. Once the system is purged, set the flow rate at 0. • Syringe, loop & injector plunger seal. g. Disconnect tubing at guard column (if present) or analytical column inlet. Non-circular drive gears provide smooth, uniform eluent delivery. The Breeze software has a Purge wizard that lets you purge the pump, 717plus Autosampler, and a 2414 Differential Refractometer. 4 Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 ml/min. Purge Solvents a. 5. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications, Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. 7. Steps to follow: Store the column in an appropriate solvent (check column information sheet) When you remove the column, add a column union so the detector gets flushed as well in the following steps. Operable in constant flow or constant. • Use less-vigorous purging • Prepare fresh mobile phase • Check pump. 6.